Panama ended the USA's long unbeaten run in Gold Cup group matches, with a 2-1 victory in Florida.
As well as bringing the hosts' 26-game undefeated streak to a close, the result secured Panama a place in the quarter-finals from Group C.
Luis Tejada and Gabriel Gomez put their side two up inside 36 minutes before Clarence Goodson pulled one back in the 66th minute.
Canada edged past 10-man Guadeloupe when Dwayne De Rosario converted a penalty in the 51st minute.
Guadeloupe had midfielder Jean-Luc Lambourde sent off in the fourth minute and could not find a response once they finally fell behind following a brave resistance.
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