Juventus have finished the International Champions Cup winless after suffering a 9-8 penalty shootout loss to Inter on Tuesday.
The two Serie A giants were playing to avoid finishing last at the eight-team friendly tournament in the United States and were locked at 1-1 after regulation time.
Ricky Alvarez had given Inter a 28th-minute lead but his opener was cancelled out by Arturo Vidal from the penalty spot on the stroke of half-time at the Sun Life Stadium in Miami Gardens.
Both teams were unable to find a winner and after a marathon penalty shootout, Inter goalkeeper Juan Pablo Carrizo was the hero; saving two spot-kicks and scoring the winner.
Inter made a bright start and created two chances thanks to Alvaro Pereira, who found space on the right of Juventus' three-man defence.
Pereira's cross in the third minute was volleyed over by the dangerous Rodrigo Palacio before the Uruguayan provided a chance for Fredy Guarin, whose tame effort was saved by Gianluigi Buffon.
When Juve settled, they looked dangerous going forward and Mirko Vucinic squandered two chances.
The Montenegro international curled a shot from just inside the area over the bar before heading an Andrea Pirlo free-kick off target when he should have done better from six yards.
The chances that followed were mostly from distance before Alvarez opened the scoring in the 28th minute.
Palacio got behind Juventus' high defensive line before setting up Guarin, whose low strike was parried by Buffon into the path of Alvarez and the Argentine tucked his finish into the bottom corner.
Inter controlled the contest after the opener without creating clear-cut opportunities before Juve made them pay.
Vidal had a penalty shout turned down in the 39th minute and just moments later, Juan fouled Vucinic with a sloppy challenge inside the area.
Vidal stepped up and put his penalty into the bottom corner, with Samir Handanovic unable to keep it out despite diving the right way.
Inter looked dangerous on the counter-attack in the second half, while Juve went close to taking the lead on the hour-mark.
Carlos Tevez squared a pass for Claudio Marchisio and the midfielder's back-flick was saved by Carrizo before he fired the rebound into the side netting.
A neat passing move led to a chance for Yuto Nagatomo but the Inter wingback's 25-yard strike lacked the power to trouble Marco Storari.
Pirlo went closest to scoring a winner in regulation time as his 30-yard free-kick struck the post in the 76th minute with Carrizo rooted to the spot.
Juventus were beaten on penalties by Everton in the first round and they tasted defeat again.
Sebastian Giovinco and Guarin had decent spot-kicks kept out by excellent stops from Carrizo and Storari respectively.
There were 16 straight goals after the two misses, with Marchisio, Andrea Ranocchia, Fernando Llorente, Alvarez, Pirlo, Mauro Icardi, Vidal, Ishak Belfodil, Angelo Ogbonna, Patrick Olsen, Martin Caceres, Marco Andreolli, Giorgio Chiellini, Pereira, Paolo De Ceglie and Jonathan all netting.
Mauricio Isla had his penalty saved by Carrizo before the Argentine goalkeeper stepped up and smashed his penalty down the middle to give Inter victory.