The Argentinian coach left out Udinese midfielder Jose Montiel and the defensive pair of Napoli's Ruben Maldonado and Libertad's Victor Caceres.

Full-backs Pablo Aguilar and Denis Caniza are also absent through injury.

Martino admitted the decisions were difficult, saying: "They all feel they have the right to a place in the squad.

"But ultimately it is the coach who has to make decisions of who makes the final cut."

Paraguay will remain in Ypane until June 19 before flying to Santa Cruz de las Sierras to play an international friendly against Bolivia on June 20.

The Guaranies will travel to Venezuela two days later to finalise their preparations for their opening match in Group C against Colombia before clashes against Argentina and the United States.

Paraguay squad: Villar (Newell's Old Boys), Bobadilla (Boca Juniors), Zayas (Bolivar); Da Silva (Toluca), Morel Rodriguez (Boca Juniors), Caceres (Tigres), Veron (Pumas), Manzur (Guarani); Bonet, Riveros (both Libertad), Vera (Liga Deportiva Universitaria), Gonzalez, Salcedo (both Cerro Porteno), Torres (San Lorenzo), Santana (Wolfsburg), Barreto (NEC Nimega), Dos Santos (Bayern Munich); Cabanas (America), Lopez (Crotone), Santa Cruz (Bayern Munich), Oscar Cardozo (Newell's Old Boys), Cuevas (America).